Cellular phenotyping of host pathogen interactions

ABSTRACT: The goal of this project is to understand the etiology and pathology of host-pathogen interaction using high-throughput cellular phenotyping. By combining the expertise in pathogen biology, informatics and stem cell biology across the institute, this project will use targeted genetic modification in mouse embryonic stem cells to identify functions of host genes in innate immunity. We will also explore the biology of mouse embryonic stem cells and their differentiation into immune competent cells. To this end we will generate homozygous and inducible knockout lines in mouse embryonic stem cells, which will be differentiated into immune competent cells, such as macrophages or dendritic cells. These will be subjected to a panel of immune challenge and stimulation assays to characterise the f

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): mus musculus

SUBMITTER:  

PROVIDER: E-ERAD-234 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress