Human transcriptome comparison in response to Staphylococcus aureus exposure

ABSTRACT: Biological replicates of THP1 cells incubated with live wild type S. aureus, THP1 cells incubated with dead wild type S. aureus and THP1 cells incubated with saeS- S. aureus were collected and their RNA were isolated via the Qiagen RNA Isolation Kit. The RNA were treated with RNAse free DNase and eluted in RNase-free water. The RNA concentration was assessed using a NanoDrop spectrophotometer, while RNA integrity (RIN >8) were obtained for all samples by Agilent 2100 Bioanalyzer. We aim to sequence 5GBp per replicate and all samples on a single lane on an Illumina platform.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER:  

PROVIDER: E-ERAD-242 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress