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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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EpiSC RNAseq

ABSTRACT: EpiSCs were transfected with different TET1 constructs or empty vector controls. RNA was collected using All prep Qiagen kit and ran on gel to check for RNA integrity.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Mouse

SUBMITTER:

PROVIDER: E-ERAD-325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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T535A

Project description:EpiCS were transfected with KLF4 and different TET1 constructs. Cells were grown in standard N2B27 medium and collected after two passages. RNA was collected using All prep Qiagen kit and ran on gel to check for RNA integrity.

2014-10-21 | E-ERAD-308 | biostudies-arrayexpress

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Project description:The requirements for self-renewal differ between EpiSCs and ES cells and the underlying mechanism is largely unknown. Here we show that mouse EpiSCs can be efficiently derived and robustly propagated even from single cells, using two small-molecule inhibitors: CHIR99021 and XAV939. The whole-genome microarray analyses is performed to confirm the identity of EpiSC maintained in CHIR/XAV by comparing the expression profile in EpiSC-CHIR/XAV to those in ESC maintained in 2i and EpiSC maintained in FGF2/activin.

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Global gene expression profile of mouse ESC-2i, EpiSC-CHIR/XAV and EpiSC-FGF2/activin

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Global expression profiling of newly derived EpiSC lines

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Project description:Epiblast stem cells (EpiSCs) maintained in the medium with activin were freed from activin to elicit neural development, exposed to various strengths of Wnt signals, which may provide the initial stage of anteroposterior regional specificities, and placed in the culture condition to arrest neural development but expand cells as neural stem cells (NSCs) with epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2).

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Identify the downstream targets of CHIR99021 and XAV939 in EpiSC using microarray analysis

Project description:The requirements for self-renewal differ between EpiSCs and ES cells and the underlying mechanism is largely unknown. Here we show that mouse EpiSCs can be efficiently derived and robustly propagated even from single cells, using two small-molecule inhibitors: CHIR99021 and XAV939. To better define how CHIR and XAV act together to maintain EpiSC self-renewal, we performed microarray analyses to identify their downstream targets. The data show the genes regulated by addition of CHIR, XAV or both.

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Global expression profiling of newly derived EpiSC lines

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