Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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DPY30 ChIP seq


ABSTRACT: H9 human embryonic stem cells (hESCs) were cultured in feeder-free chemically-defined conditions in medium containing 10ng/ml Activin A and 12ng/ml FGF2 (Vallier L. 2011, Methods in Molecular Biology, 690: 5766). Chromatin immunoprecipitation was performed as described in Brown S. et al. 2011. Stem Cells 29: 117685 by using 5ug of anti-DPY30 antibody (Sigma, cat. number HPA043761). This protocol was performed in control hESCs (expressing a scrambled shRNA) and in hESCs stably expressing an shRNA against DPY30 (Sigma, clone n. TRCN0000131112).This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER:  

PROVIDER: E-ERAD-365 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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