Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

10 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome profiling of zebrafish embryos from the SAT Sanger AB T bingen strain

ABSTRACT: Total RNA was extracted from zebrafish embryos from the SAT (Sanger AB Tbingen) strain. The RNA was DNase treated. Stranded RNAseq libraries were constructed using the Illumina TruSeq Stranded RNA protocol after treatmant with Ribozero.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Danio rerio

SUBMITTER:

PROVIDER: E-ERAD-421 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome profiling of zebrafish embryos from the SAT Sanger AB Tubingen strain 2

Project description:Total RNA was extracted from zebrafish embryos from the SAT (Sanger AB Tubingen) strain. The RNA was DNase treated. The 3' ends of fragmented RNA was pulled down using polyT oligos attached to magnetic beads, reverse transcribed, made into Illumina libraries and sequenced using IlluminaHiSeq paired-end sequencing. Protocol: Total RNA was extracted and DNase treated. Fragmented RNA was enriched for the 3 ends by pull down using a polyT oligo attached to magnetic beads. An RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5 end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5 end followed by 12 random bases, then an 8 base indexing tag, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by PCR. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2016-01-12 | E-ERAD-425 | biostudies-arrayexpress

RNASeq of zebrafish embryos infected with pathogens

Project description:Total RNA was extracted from zebrafish embryos at different time points post pathogen infection. The RNA was DNase treated. Stranded RNAseq libraries were constructed using the Illumina TruSeq Stranded RNA protocol with oligo dT pulldown. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2016-02-09 | E-ERAD-428 | biostudies-arrayexpress

Transcriptome profiling of zebrafish tcf3a and tcf3b knockout embryos

Project description:Total RNA was extracted from zebrafish embryos at 80% epiboly and genotyped for tcf3a and tcf3b to identify wild type, heterozygous and homozygous knockout embryos. The RNA was DNase treated. Stranded RNAseq libraries were constructed using the Illumina TruSeq Stranded RNA protocol with oligo dT pulldown.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2016-01-19 | E-ERAD-416 | biostudies-arrayexpress

Baseline expression from transcriptional profiling of zebrafish developmental stages

Project description:Establish baseline expression transcriptional signatures for zebrafish developmental stages. Total RNA was extracted from single wild type zebrafish embryos at a series of different developmental stages. RNA from 12 embryos was pooled to generate a replicate.The RNA was DNase treated. Stranded RNAseq libraries were constructed using the Illumina TruSeq Stranded RNA protocol with oligo dT pulldown.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2016-06-12 | E-ERAD-475 | biostudies-arrayexpress

ZF adult transcriptome

Project description:Total RNA was extracted from adult male and female zebrafish. Illumina cDNA libraries were prepared from polyA+ RNA and sequenced using Illumina HiSeq paired-end sequencing. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ protocol: Total RNA was extracted from zebrafish embryos using Trizol and DNase treated. Libraries were made using the Illumina cDNA protocol from polyA+ RNA.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2013-01-07 | E-ERAD-54 | biostudies-arrayexpress

RNA sequencing of mouse haemopoietic cells BCMX ASMX SMMX HNMX DPMX DTMX

Project description:This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2014-10-06 | E-ERAD-304 | biostudies-arrayexpress

ZFP106 expression profile

2013-03-11 | E-ERAD-111 | biostudies-arrayexpress

Colistin resistance of Acinetobacter baumannii with RNAseq

Project description:These data are part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2015-08-04 | E-ERAD-375 | biostudies-arrayexpress

mouse embryonic development

2016-01-12 | E-ERAD-447 | biostudies-arrayexpress