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Transcriptome sequencing of Hymenolepis microstoma a classic laboratory model in cestodology

ABSTRACT: Hymenolepis spp. (H. diminuta, H. nana and H. microstoma) are rodent-hosted tapeworms (Platyhelminthes: Cestoda) that have been used as laboratory and teaching models since the 1950s, and consequently much of our understanding of the basic physiology, biochemistry and anatomy of tapeworms in general stems from research using these species. As representatives of the order Cyclophyllidea, they are closely related to species with significant medical and economic importance such as Taenia and Echinococcus spp., but unlike these may be maintained in vivo using only laboratory mice and flour beetles (n.b. Echinoccous spp. are hosted by foxes and Taenia spp. are hosted by pigs or cows). This effort brings a classical laboratory model into the genomic age, allowing researchers in silico access to its genome and expressed gene transcripts and thereby greatly expediting research directed at understanding the genetic basis of tapeworm biology.

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Hymenolepis microstoma

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PROVIDER: E-ERAD-56 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:N6-methyladenosine (m6A) is the methylation of adenosine at position N6 and is notably one of the most abundant modifications found in mRNA of eukaryotes. Although it does not interrupt the coding functions of transcripts, it assists in regulating several RNA functions like splicing, stability, translocation and translation and is associated with disease development, cell proliferation, cell migration, cell invasion, autophagy, cell apoptosis, and drug resistance. In this study, we performed a colorimetric assay, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and methylated RNA immunoprecipitation sequencing (MeRIP-seq) to assess the RNA N6-methyladenosine landscape of Echinococcus granulosus; a tapeworm species of the phylum Platyhelminthes that is responsible for a cosmopolitan zoonosis (cystic echinococcosis) of public health importance. While the levels of m6A modification were not significantly different between the life cycle stages, many important genes including notably enriched and expanded protein domains like Dyneins, Hsp70, and Cadherin thought to play important roles in adaptation to parasitism were m6A-modified. These genes were functionally enriched in biological processes like cell division, cell adhesion, microtubule-based movement, important pathways involved in parasitism (e.g. MAPK), and disease processes. Additionally, the m6A alterations were present in the first exon of modified genes, suggesting gene regulatory activities. Conclusively, m6A modification of the mRNA is present in Echinococcus, and is mediated by orthologues of known mammalian methyltransferases but possibly irreversible due to the absence of demethylases. The demonstration of differentially m6A-methylation in the different life cycle stages of Echinococcus suggests a role in the development and survival of the tapeworm. It also provides the groundwork for future studies in understanding the implication of RNA methylation in the treatment of echinococcoses.

Dataset Information

Transcriptome sequencing of Hymenolepis microstoma a classic laboratory model in cestodology

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