DNA sequence at CpG islands directly influences chromatin structure via the CpG binding protein Cfp1

ABSTRACT: CpG islands (CGIs) are prominent in the mammalian genome due to their G+C rich base composition and high density of CpG dinucleotides. Most mammalian gene promoters are embedded within CGIs that lack DNA methylation and coincide with sites of histone H3 lysine 4 trimethylation (H3K4me3),irrespective of transcriptional activity. In spite of these intriguing correlations, the functional significance of non-methylated CGI sequences with respect to chromatin structure and transcription is unknown. High throughput sequencing of Cfp1-bound chromatin identified a striking concordance with non-methylated CGIs and sites of H3K4me3 in the mouse brain. Protocol: Brains were manually homogenised to a single cells suspension and crosslinked using 1% formaldehyde. Chromatin was sheared using a combinati

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): mus musculus

SUBMITTER:  

PROVIDER: E-ERAD-79 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress