Changes in gene expression during treatment with Melatonin
Ontology highlight
ABSTRACT: This study is focused to find new genes involved in the Melatonin signaling in osteoblast. We will use primary cells that are pre-osteoblast lineage; our aim is to analize the transcriptome during several stages, such as proliferation, differentiation and mineralization. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:This study is designed to identify gene promoters and enhancers target of foxo1, an transcription factor activated by melatonin in osteoblast cells. Protocol:1. Primary osteoblast cells were grown at 70-80% confluency in 150mm culture dishes.2. These cells were then treated with melatonin for 24h under serum-deprived condition.3. After the completion of treatment cells were fixed with medium containing formaldehyde, washed with PBS and reaction was stopped by adding glycine stop solution. Cells were collected in cell scraping solution. 4. Further sonication and CHIP reaction with foxo1 antibody was performed using Active Motif CHIP-IT express kit following manufacturers instructions.5. After CHIP DNA was purified using Active Motif Chromatin IP DNA purification Kit following manufacturers instructions. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:http://www.sanger.ac.uk/resources/downloads/bacteria/salmonella.htmlThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:http://www.sanger.ac.uk/resources/downloads/bacteria/citrobacter-rodentium.htmlThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:http://www.sanger.ac.uk/resources/downloads/bacteria/clostridium-difficile.htmlThese data are part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:http://www.sanger.ac.uk/resources/downloads/bacteria/klebsiella-pneumoniae.htmlThese data are part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:http://www.sanger.ac.uk/resources/downloads/bacteria/ This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:http://www.sanger.ac.uk/resources/downloads/bacteria/streptococcus-suis.htmlThese data are part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:http://www.sanger.ac.uk/resources/downloads/bacteria/klebsiella-pneumoniae.htmlThese data are part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:http://www.sanger.ac.uk/resources/downloads/bacteria/These data are part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:http://www.sanger.ac.uk/resources/downloads/bacteria/These data are part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/