Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Pseudomonas aeruginosa strain PA01 grown in swimming of swarming conditions

ABSTRACT: Microarray were done on 4 independent cultures. PA01 was grown for 6 hours to late log growth phase (OD600 0.9-1.0) in liquid BM2-swarming media under shaking conditions(swimming) or for 18 hours at 37C on BM2-swarming plate containing 0.5% (w/v) agar and 0.1% casamino acids. Cells were harvested either by centrifugation(swimming) or by a sterile inoculating loop (swarming) and were resuspended n BM2-swarming media supplement with RNAprotect reagent (Qiagen)

INSTRUMENT(S): ScanArray [PerkinElmer]

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Manjeet Bains 

PROVIDER: E-FPMI-10 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Swarming of Pseudomonas aeruginosa is a complex adaptation leading to increased production of virulence factors and antibiotic resistance.

Overhage Joerg J   Bains Manjeet M   Brazas Michelle D MD   Hancock Robert E W RE  

Journal of bacteriology 20080201 8

In addition to exhibiting swimming and twitching motility, Pseudomonas aeruginosa is able to swarm on semisolid (viscous) surfaces. Recent studies have indicated that swarming is a more complex type of motility influenced by a large number of different genes. To investigate the adaptation process involved in swarming motility, gene expression profiles were analyzed by performing microarrays on bacteria from the leading edge of a swarm zone compared to bacteria growing in identical medium under s  ...[more]

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