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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human monocytic cells THP-1 stimulated with LPS (10ng/ml) in presence or absence of LL-37 (5 ug/ml), as well as with the peptide alone, for 4 hours

ABSTRACT: The objective of the study was to evaluate transcriptional response of endotoxin-stimulated human monocytic cells in presence or absence of host defense peptide LL-37 at low physiologically relevant concentrations. Human monocytic cells THP-1 were stimulated with LPS (10ng/ml) in presence or absence of LL-37 (5 ug/ml), as well as with the peptide alone, for 4 hours. The trends of LPS-induced altered gene expression in presence of the peptide were further validated by quantitative real-time PCR.

ORGANISM(S): Homo sapiens

SUBMITTER: Neeloffer Mookherjee

PROVIDER: E-FPMI-6 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Bovine and human cathelicidin cationic host defense peptides similarly suppress transcriptional responses to bacterial lipopolysaccharide.

Mookherjee Neeloffer N Wilson Heather L HL Doria Silvana S Popowych Yurij Y Falsafi Reza R Yu Jie Jessie JJ Li Yuexin Y Veatch Sarah S Roche Fiona M FM Brown Kelly L KL Brinkman Fiona S L FS Hokamp Karsten K Potter Andy A Babiuk Lorne A LA Griebel Philip J PJ Hancock Robert E W RE

Journal of leukocyte biology 20060830 6

Genomic approaches can be exploited to expose the complexities and conservation of biological systems such as the immune network across various mammalian species. In this study, temporal transcriptional expression profiles were analyzed in human and bovine monocytic cells in response to the TLR-4 agonist, LPS, in the presence or absence of their respective host defense peptides. The cathelicidin peptides, human LL-37 and bovine myeloid antimicrobial peptide-27 (BMAP-27), are homologs, yet they h ...[more]

PMID: 16943385

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