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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human CD14+ monocytes treated with innate defence regulator peptide IDR-1 in the presence/absence of lipopolyssacharide

ABSTRACT: Effect of peptide IDR-1 on CD14+ human monocytes. >br< >br< Effect of peptide IDR-1 on LPS-induced responses in CD14+ human monocytes.>br< >br< Human peripheral blood mononuclear cells (PBMC) were treated with innate defence regulator peptide IDR-1 in the presence and absence of LPS (purified from Pseudomonas aeruginosa) for 4hrs. The objective was to obtain a transcriptional profile of responses induced by IDR-1 and the effect of IDR-1 on LPS-induced transcription.

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Homo sapiens

SUBMITTER: Neeloffer Mookherjee

PROVIDER: E-FPMI-8 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An anti-infective peptide that selectively modulates the innate immune response.

Scott Monisha G MG Dullaghan Edie E Mookherjee Neeloffer N Glavas Natalie N Waldbrook Matthew M Thompson Annick A Wang Aikun A Lee Ken K Doria Silvana S Hamill Pam P Yu Jie Jessie JJ Li Yuexin Y Donini Oreola O Guarna M Marta MM Finlay B Brett BB North John R JR Hancock Robert E W RE

Nature biotechnology 20070325 4

We show that an innate defense-regulator peptide (IDR-1) was protective in mouse models of infection with important Gram-positive and Gram-negative pathogens, including methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus and Salmonella enterica serovar Typhimurium. When given from 48 h before to 6 h after infection, the peptide was effective by both local and systemic administration. Because protection by IDR-1 was prevented by in vivo depletion of monocytes and macrop ...[more]

PMID: 17384586

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