Transcription profiling of Drosophila EcR-dependent gene expression in MB neurons at the onset of axon pruning

ABSTRACT: This study identifies genes that show EcR-dependent gene expression in MB neurons at the onset of axon pruning. Experiment Overall Design: A dominant negative form of the ecdysone receptor (EcRDN) was expressed in MB neurons by OK107-GAL4. Animals were staged at 0 and 5 hours relative to puparium formation. RNA was isolated from MB neurons labeled with mCD8::GFP driven by OK107-GAL4 by laser capture microdissection, labeled and hybridized to Affymetrix Drosophila Genome Arrays. These microarrays were compared to RNA isolated from y,w animals at similar time points.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Eric Hoopfer 

PROVIDER: E-GEOD-10013 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress