Transcription profiling of Plasmodium falciparum after exposing drug-selected mutants to short term CQ treatment

ABSTRACT: Mutations in PfCRT confer chloroquine (CQ) resistance in P. falciparum. Point mutations in the homolog of the mammalian multidrug resistance gene (pfmdr1) can also modulate the levels of CQ response. However, parasites with the same pfcrt and pfmdr1 alleles exhibit a wide range of drug sensitivity, suggesting that additional genes contribute to levels of CQ resistance (CQR). We used 3 isogenic lines which have different drug resistance profiles corresponding to unique mutations in the pfcrt gene (106/1K76, 106/176I, and 106/76I-352K) to study changes in gene expression with and without CQ and genomic variations, i.e. copy number (CN) changes. RNA transcription levels from 45 genes were significantly altered in one or both mutants relative to the parent line. Of particular interest are gene

ORGANISM(S): Plasmodium falciparum

SUBMITTER: Hongying Jiang 

PROVIDER: E-GEOD-10022 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress