Transcription profiling of human 3D cultured chrondrocytes treated with supernatant of synovial fibroblasts from an RA patients vs controls to investigate key regulatory molecules of cartilage destruction in rheumatoid arthritis

ABSTRACT: We have studied the expression profile of 3D cultured human chondrocytes that were stimulated with supernatant of synovial fibroblasts derived from a RA patient (RASF=HSE cell line) and from a normal donor (NDSF=K4IM cell line), respectively. For this purpose, passage 2 human chondrocytes were cultured for 14 days in alginate beads and subsequently stimulated for 48 hours with supernatant of RASF and NDSF. Baseline expression was determined of unstimulated chondrocytes. Differential genome-wide microarray analysis of RASF and NDSF stimulated chondrocytes disclosed a distinct expression profile related to cartilage destruction involving marker genes of inflammation (COX-2), NF-kappa B signaling pathway (TLR2), cytokines/chemokines and receptors (CXCL1-3, CCL20, CXCL8, CXCR4, IL-6, IL-1beta

ORGANISM(S): Homo sapiens

SUBMITTER: Thomas Häupl 

PROVIDER: E-GEOD-10024 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress