MRNA expression profiling of mouse microvasculature

ABSTRACT: Combination of reverse- and chemical genetic screens reveals a network of novel angiogenesis inhibitors and targets Drug target identification and validation are bottlenecks in the drug discovery process. Accordingly there is a need to develop new methods to facilitate the development of much-needed innovative drugs. We have combined reverse- and chemical genetics to identify new targets modulating blood vessel development. Through mRNA expression profiling in mice we identified 155 drugable gene products that were enriched in the microvasculature. Orthologs of 50 of these candidates were knocked down in a reverse genetic screen in zebrafish. 16 of the 50 genes encoded products that affected angiogenesis. In parallel, screening of 300 known drugs and pharmacologically active compounds i

ORGANISM(S): Mus musculus

SUBMITTER: Erik Billgren 

PROVIDER: E-GEOD-10035 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress