Identification of human minor H antigens based on genotyping of pooled DNA

ABSTRACT: Minor histocompatibility (H) antigens are the molecular targets of allo-immunity responsible both for the development of anti-tumor effects and for graft-versus-host disease (GVHD) in allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, despite their potential clinical use, our knowledge of human minor H antigens is largely limited by the lack of efficient methods of their characterization. Here we report a robust and efficient method of minor H gene discovery that combines whole genome association scans (WGAS) with cytotoxic T-lymphocyte (CTL) assays, in which the genetic loci of minor H genes recognized by the CTL clones are precisely identified using pooled-DNA analysis of immortalized lymphoblastoid cell lines with/without susceptibility to those CTLs. Using this

ORGANISM(S): Homo sapiens

SUBMITTER: Yasuhito Nannya 

PROVIDER: E-GEOD-10044 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress