Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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A compendium of antibiotic-induced transcription profiles reveals broad regulation of P. multocida virulence genes

ABSTRACT: Treatment of bacteria with antibiotics at or close to the inhibitory concentration leads to specific transcriptional responses often affecting target genes and targets pathways. A dataset of transcriptional profiles (compendium) induced by antibiotics with known mode-of-action (MoA) can be used to gain information on the putative MoA of novel substances with unknown MoAs. We used a Pasteurella multocida microarray to generate a compendium of transcriptional profiles and to obtain information on the putative MoA of a novel antibiotic compound. We also show a strong impact of the bacteriostatic antibiotics on P. multocida virulence gene transcription. Keywords: antibiotica treatment, time course Midlog-grown cultures of P. multocida were treated for 10 or 30 min with 8 different antibiotics and one novel compound (thiazin) at minimal inhibitory concentrations (MICs) and were harvested. Control bacteria were not-treated and harvested at approximately the same optical density an OD578 of ~ 0.5. Total RNA was extracted from these samples and labelled with biotin. P. multocida whole genome transcriptional profiling was performed by hybridization on the custom-made Affymetrix microarray according to the manufacturer’s instructions. The experiments were done in triplicates.

ORGANISM(S): Pasteurella multocida

SUBMITTER: Paul Selzer 

PROVIDER: E-GEOD-10051 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A compendium of antibiotic-induced transcription profiles reveals broad regulation of Pasteurella multocida virulence genes.

Melnikow E E   Schoenfeld C C   Spehr V V   Warrass R R   Gunkel N N   Duszenko M M   Selzer P M PM   Ullrich H J HJ  

Veterinary microbiology 20080329 3-4

The transcriptional responses of Pasteurella multocida to eight antibiotics with known mode of actions (MoAs) and one novel antibiotic compound with an unknown MoA were collected to create a compendium of transcriptional profiles for MoA studies. At minimal inhibitory concentration the three bactericidal compounds enrofloxacin, cefquinome and the novel compound had a minor impact on gene regulation with approximately 1% of the P. multocida genome affected, whilst the bacteriostatic compounds flo  ...[more]

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