Transcriptional Analysis of 1M Regions in Medicago truncatula Using Tiling Microarrays

ABSTRACT: We report here a transcriptonal analysis in six different organ types of a approximately 1 Mb region in legume plants barrel medic (Medicago truncatula) which is sytenic with soybean (Glycine max). We used oligonucleotide tiling microarrays to detecte transcription of over 80% of the predicted genes in both species. We detected differential gene expression in the six examined organ types. Keywords: RNA Both the barrel medic and soybean tiling arrays were produced on the Maskless Array Synthesizer platform. Briefly, tiling-paths consisting of 36-mer oligonucleotides offset by five nucleotides were designed to represent both DNA strands of the selected barrel medic and soybean genome sequence. Probes were synthesized at a feature-density of 390,000 probes per array in a “chess board” des

ORGANISM(S): Medicago truncatula

SUBMITTER: XingWang Deng 

PROVIDER: E-GEOD-10055 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress