Transcription profiling of mouse liver from wild-type or genetically deleted AHRs were treated with corn oil vehicle or TCDD reveals aryl hydrocarbon receptor regulates distinct dioxin-dependent and dioxin-independent gene batteries

ABSTRACT: Conventional biochemical and molecular techniques identified previously several genes whose expression is regulated by the aryl hydrocarbon receptor (AHR). We sought to map the complete spectrum of AHR-dependent genes in male adult liver using expression arrays to contrast mRNA profiles in Ahr-null mice (Ahr–/–) with those in mice with wild-type AHR (Ahr+/+). Transcript profiles were determined both in untreated mice and in mice treated 19 h earlier with 1000 µg/kg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Expression of 456 ProbeSets was significantly altered by TCDD in an AHR-dependent manner, including members of the classic AHRE-I gene battery, such as Cyp1a1, Cyp1a2, Cyp1b1, and Nqo1. In the absence of exogenous ligand, AHR status alone affected expression of 392 ProbeSets, sugg

ORGANISM(S): Mus musculus

SUBMITTER: Paul Boutros 

PROVIDER: E-GEOD-10082 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress