Metatranscriptomes of phytoplankton blooms from an ocean acidification mesocosm experiment

ABSTRACT: Sequencing the metatranscriptome can provide information about the response of organisms to varying environmental conditions. We present a methodology for obtaining random whole-community mRNA from a complex microbial assemblage using Pyrosequencing. The metatranscriptome had, with minimum contamination by ribosomal RNA, significant coverage of abundant transcripts, and included significantly more potentially novel proteins than in the metagenome. Keywords: metatranscriptome, mesocosm, ocean acidification This experiment is part of a much larger experiment. We have produced 4 454 metatranscriptomic datasets and 6 454 metagenomic datasets. These were derived from 4 samples. The experiment is an ocean acidification mesocosm set up in a Norwegian Fjord in 2006. We suspended 6 bags containing

ORGANISM(S): marine metagenome

SUBMITTER: Jack Gilbert 

PROVIDER: E-GEOD-10119 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress