ChIP-chip analysis of HTZ-1 and RNA Polymerase II in wildtype (N2) embryos

ABSTRACT: In all eukaryotes, histone variants are incorporated into a subset of nucleosomes to create functionally specialized regions of chromatin. One such variant, H2A.Z, replaces histone H2A and is required for viability in all metazoans tested to date. However, the function of H2A.Z in chromatin organization, transcription, and development remains controversialunclear. We mapped the genome-wide distribution of the C. elegans H2A.Z ortholog HTZ-1 during embryogenesis by Chromatin ImmunoPrecipitation on DNA microarrays (ChIP-chip). We find that H2A.Z is incorporated upstream of approximatelybout 25% of C. elegans genes, preferentially upstream of genes required for development and occupied by RNA polymerase II. Fewer sites of HTZ-1 localization occur on the X chromosome relative to autosomes, con

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Jason Lieb 

PROVIDER: E-GEOD-10201 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress