Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Genetic homogeneity among strains of Clostridium botulinum type A1 with a unique toxin gene cluster organization

ABSTRACT: Comparative genomic hybridizations were performed to compare bont/A1 strains with an A2-like toxin gene cluster organization to the genome sequenced strain, C. botulinum ATCC 3502. Keywords: comparative genomic hybridization For each hybridization, the reference strain (C. botulinum ATCC 3502) was labeled with Cy5 and each test strain was labeled with Cy3.

ORGANISM(S): Clostridium botulinum

SUBMITTER: Mark Jacobson

PROVIDER: E-GEOD-10223 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic homogeneity of Clostridium botulinum type A1 strains with unique toxin gene clusters.

Raphael Brian H BH Luquez Carolina C McCroskey Loretta M LM Joseph Lavin A LA Jacobson Mark J MJ Johnson Eric A EA Maslanka Susan E SE Andreadis Joanne D JD

Applied and environmental microbiology 20080523 14

A group of five clonally related Clostridium botulinum type A strains isolated from different sources over a period of nearly 40 years harbored several conserved genetic properties. These strains contained a variant bont/A1 with five nucleotide polymorphisms compared to the gene in C. botulinum strain ATCC 3502. The strains also had a common toxin gene cluster composition (ha-/orfX+) similar to that associated with bont/A in type A strains containing an unexpressed bont/B [termed A(B) strains]. ...[more]

PMID: 18502928

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Project description:Profound understanding of the mechanisms foodborne pathogenic bacteria utilize in adaptation to the environmental stress they encounter during food processing and storage is of paramount importance in design of control measures. Chill temperature is a central control measure applied in minimally processed foods; however, data on the mechanisms the foodborne pathogen Clostridium botulinum activates upon cold stress are scarce. Global gene expression analysis on the C.M-BM- botulinum ATCC 3502 strain upon temperature downshift from 37 M-BM-0C to 15M-BM- M-BM-0C was performed to identify the cold-responsive gene set of this organism. Significant up- or down-regulation of 16 and 11 genes, respectively, was observed already 1 h after the cold shock. At 5 h after the temperature downshift, 199 and 210 genes were up- and down-regulated, respectively. Thus, the cold shock rapidly affected the expression of a gene set of a relatively small size, indicating a targeted acute response to cold shock, whereas extensive metabolic remodeling took place after prolonged exposure to cold. Induction of genes related to fatty acid biosynthesis, oxidative stress response, and iron uptake and storage was observed, in addition to mechanisms previously characterized as cold-tolerance related in bacteria. Furthermore, induction of several uncharacterized DNA-binding transcriptional regulator-encoding genes was observed, suggesting involvement of novel regulatory mechanisms in the cold shock response of C.M-BM- botulinum. The role of such regulatory proteins, CBO0477 and CBO0558A, in cold tolerance of C.M-BM- botulinum ATCC 3502 was demonstrated by the deteriorated growth of mutants of the respective genes at 17M-BM- M-BM-0C. C. botulinum ATCC 3502 wild type cold-shocked vs. pre-cold-shock; 3 replicates; growth at 37C in TPGY broth batch culture and subjected to cold shock to 15C; sampling at mid-log growth phase before cold shock, and 1 h and 5 h after temperature downshift to 15C (= 3 time points). Dye-swapped hybridization.

Dataset Information

Genetic homogeneity among strains of Clostridium botulinum type A1 with a unique toxin gene cluster organization

Publications

Genetic homogeneity of Clostridium botulinum type A1 strains with unique toxin gene clusters.

Similar Datasets

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