Transcription profiling of mouse IRF4,8 null pre-B cells treated with ILF7 reveals convergent molecular pathways that induce immunoglobulin light-chain recombination

ABSTRACT: Productive rearrangement of the immunoglobulin heavy chain locus triggers a major developmental checkpoint that promotes limited clonal expansion of pre-B cells, culminating in cell cycle arrest and rearrangement of the kappa (κ) or lambda (λ) light-chain loci. B lineage cells lacking the related transcription factors IRF-4 and IRF-8 undergo a developmental arrest at the cycling pre-B cell stage and are blocked for light-chain recombination. Using Irf-4,8-/- pre-B cells we demonstrate that two pathways converge to synergistically drive light-chain rearrangement, a process that is not simply activated by cell cycle exit. One pathway is directly dependent on IRF-4, whose expression is elevated by pre-BCR signaling. IRF-4 targets the κ 3′ and λ enhancers to increase locus accessibilit

ORGANISM(S): Mus musculus

SUBMITTER: Harinder Singh 

PROVIDER: E-GEOD-10273 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress