Endothelial in vivo vs. in vitro responses to LPS
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: John Maresh
PROVIDER: E-GEOD-1044 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
In vivo transcriptional response of cardiac endothelium to lipopolysaccharide.
Maresh J Gregory JG Xu Huaxia H Jiang Nan N Shohet Ralph V RV
Arteriosclerosis, thrombosis, and vascular biology 20040819 10
<h4>Objective</h4>Vascular endothelial cells must integrate stimuli from multiple sources, including plasma, leukocytes, and neighboring components of the vessel. These stimuli are difficult to recapitulate in vitro. We have developed a method to examine the in vivo regulation of gene expression in endothelial cells and have applied it to a model of sepsis.<h4>Methods and results</h4>We used fluorescent-activated cell sorting to isolate highly purified endothelial cells from the hearts of transg ...[more]
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