Transcription profiling of human dental pulp stem cells in long term culture

ABSTRACT: In previous studies, human dental pulp stem cells (hDPSCs) were mainly isolated from adults. In this manuscript, we tried characterization of hDPSCs isolated from an earlier developmental stage to evaluate potential usage of these cells for tissue regenerative therapy. hDPSCs isolated at the crown-completed stage showed a higher proliferation rate than those isolated at the later stage. When the cells from either group were cultured in medium promoting differentiation towards cells of the osteo/odontoblastic lineage, both became alkaline phosphatase positive, produced calcified matrix, and were also capable of forming dentin-like matrix on scaffolds in vivo. However, during long-term passage, these cells underwent a change in morphology and lost their differentiation ability. The results o

ORGANISM(S): Homo sapiens

SUBMITTER: Ken-ichi Tezuka 

PROVIDER: E-GEOD-10444 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress