Using ChIP-chip technology to reveal common principles of transcriptional repression in normal and cancer cells

ABSTRACT: We compared 12 different cell populations, including embryonic stem cells before and during differentiation into embryoid bodies as well as various types of normal and tumor cells to determine if pluripotent vs. differentiated cell types use different mechanisms to establish their transcriptome. We first identified genes that were not expressed in the 12 different cell populations and then determined which of them were regulated by histone methylation, DNA methylation, at the step of productive elongation, or by the inability to establish a pre-initiation complex. For these experiments, we performed chromatin immunoprecipitation using antibodies to H3me3K27, H3me3K9, 5-methyl cytosine, and POLR2A. We found that a) the percentage of low expressed genes bound by POLR2A, H3me3K27, H3me3K9, or

ORGANISM(S): Mus musculus

SUBMITTER: Vitalina Komashko 

PROVIDER: E-GEOD-10504 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress