Specific Roles for the Ccr4-Not Complex Subunits in Expression of the Genome

ABSTRACT: These Affymetrix data were used to determine the role of each non-essential subunit of the conserved Ccr4-Not complex in the control of gene expression in the yeast S. cerevisiae. The study was performed with cells growing exponentially in high glucose and with cells grown to glucose depletion. Specific patterns of gene de-regulation were observed upon deletion of any given subunit, revealing the specificity of each subunit’s function. Consistently, the purification of the Ccr4-Not complex through Caf40p by tandem affinity purification from wild-type cells or cells lacking individual subunits of the Ccr4-Not complex revealed that each subunit had a particular impact on complex integrity. Furthermore, the micro-arrays revealed that the role of each subunit was specific to the growth conditi

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Martine Collart 

PROVIDER: E-GEOD-10521 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress