Transcription profiling of chicken to investigate regulation of the early embryonic RPE (retinal pigment epithelium) transcriptome by the neural retina

ABSTRACT: Purpose: The retinal pigment epithelium (RPE) forms the outer blood-retinal barrier. Primary cultures of RPE can model the barrier, but are very sensitive to culture conditions. We examined how the neural retina regulates the RPE transcriptome in a culture model of embryonic development. Attention focused on the tight junctional genes essential for barrier function. Methods: Chick RPE from embryonic day 7 (E7) or embryonic day 14 (E14) was cultured on filters in a serum free medium. Media conditioned by organ culture of neural retinas was applied to the apical surface of the cultured RPE. Fetal bovine serum (2%) was included in some experiments. When the transepithelial electrical resistance (TER) reached a plateau, total RNA was isolated to probe the chick genome on Affymetrix microarra

ORGANISM(S): Gallus gallus

SUBMITTER: Lawrence Rizzolo 

PROVIDER: E-GEOD-10538 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress