Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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U937 cells: differentiating vs. undifferentiated

ABSTRACT: Gene-expression changes in U937 cells undergoing monocytic differentiation with TPA treatment Keywords: differentiation treatment The promonocytic U937 cell line was maintained at 37C in RPMI 1640 medium (Mediatech) supplemented with 10% fetalclone (HyClone) under a 5% CO2, 95% air atmosphere. U937 cells were differentiated by the addition to the growth medium 100 nM 12-O-tetradeconoyl-phorbol 13-acetate (TPA) (Sigma).Three condition experiment, treated with TPA for two different time points (27 and 96 hrs) vs. untreated. Biological replicates: independently grown, treated and harvested

ORGANISM(S): Homo sapiens

SUBMITTER: Elizaveta Benevolenskaya 

PROVIDER: E-GEOD-10560 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation.

Lopez-Bigas Nuria N   Kisiel Tomasz A TA   DeWaal Dannielle C DC   Holmes Katie B KB   Volkert Tom L TL   Gupta Sumeet S   Love Jennifer J   Murray Heather L HL   Young Richard A RA   Benevolenskaya Elizaveta V EV  

Molecular cell 20080801 4

Retinoblastoma protein (pRB) mediates cell-cycle withdrawal and differentiation by interacting with a variety of proteins. RB-Binding Protein 2 (RBP2) has been shown to be a key effector. We sought to determine transcriptional regulation by RBP2 genome-wide by using location analysis and gene expression profiling experiments. We describe that RBP2 shows high correlation with the presence of H3K4me3 and its target genes are separated into two functionally distinct classes: differentiation-indepen  ...[more]

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