Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin

ABSTRACT: ChIP-chip analyses of ClrC components across the S. pombe genome. Keywords: ChIP-chip Chromatin immunoprecipitated DNA recovered with antibody against FLAG-tagged protein from wild-type asynchronous growing fission yeast cells and whole cell extracts DNA were amplified by random priming and respectively labeled with Cy5 and Cy3. Labeled DNA samples were mixed and hybridized onto a 44k pombe Agilent oligo array. Data were processed using Agilent scanner and Feature Extraction software

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Shiv Grewal

PROVIDER: E-GEOD-10561 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin.

Zhang Ke K Mosch Kerstin K Fischle Wolfgang W Grewal Shiv I S SI

Nature structural & molecular biology 20080316 4

Heterochromatin assembly, involving methylation of histone H3 lysine 9 (H3K9me), regulates various chromosomal processes. In fission yeast, heterochromatin targeted to specific repeat loci in an RNAi-dependent manner spreads across extended domains to exert regional epigenetic control. The Clr4 methyltransferase complex (ClrC) is responsible for nucleation and spreading of heterochromatin; however, its recruitment to heterochromatic repeats is poorly understood. Here we demonstrate that ClrC com ...[more]

PMID: 18345014

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Dataset Information

Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin

Publications

Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin.

Similar Datasets

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