SiRNA silencing of p63 (trp63) in human primary keratinocytes via siRNA oligo transfection.

ABSTRACT: Genome-wide identification of bona fide targets of transcription factors in mammalian cells is still a challenge. We present a novel integrated computational and experimental approach to identify direct targets of a transcription factor. This consists in measuring time-course (dynamic) gene expression profiles upon perturbation of the transcription factor under study, and in applying a novel reverse-engineering” algorithm (TSNI) to rank genes according to their probability of being direct targets. Using primary keratinocytes as a model system, we identified novel transcriptional target genes of Trp63, a crucial regulator of skin development. TSNI-predicted Trp63 target genes were validated by Trp63 knockdown and by ChIP-chip to identify Trp63-bound regions in vivo. Our study revealed tha

ORGANISM(S): Mus musculus

SUBMITTER: Diego di Bernardo 

PROVIDER: E-GEOD-10564 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress