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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Placental gene expression in severe preeclampsia.

ABSTRACT: The series is composed of thirty hybridizations for analysis of differentially expressed genes in normal placenta tissues from patients with normal labor and placenta tissues from patients with severe preeclampsia. Patients tissues samples were obtained from (Vasilis. Total RNA extraction was performed by using the MagNa Pure Compact RNA Isolation Kit (Roche Applied Science). Keywords: Gene expression study, Disease state Twenty-six out of 50 normal placentas were randomly selected to match with 17 preeclamptic placentas, accounting for parity. Microarrays were performed applying a direct comparison design.

ORGANISM(S): Homo sapiens

SUBMITTER: Vasilis Sitras

PROVIDER: E-GEOD-10588 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential placental gene expression in severe preeclampsia.

Sitras V V Paulssen R H RH Grønaas H H Leirvik J J Hanssen T A TA Vårtun A A Acharya G G

Placenta 20090226 5

We investigated the global placental gene expression profile in severe preeclampsia. Twenty-one women were randomly selected from 50 participants with uncomplicated pregnancies to match 21 patients with severe preeclampsia. A 30K Human Genome Survey Microarray v.2.0 (Applied Biosystems) was used to evaluate the gene expression profile. After RNA isolation, five preeclamptic placentas were excluded due to poor RNA quality. The series composed of 37 hybridizations in a one-channel detection system ...[more]

PMID: 19249095

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