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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human peripheral blood mononuclear cells isolated from LGL leukemia samples reveals role of sphingolipid signaling of cytotoxic lymphocytes

ABSTRACT: We used microarrays to expression profile peripheral blood mononuclear cells (PBMCs) from LGL leukemia patients and control subjects to identify survival pathways that render leukemic LGL resistant to activation induced cell death. Experiment Overall Design: Leukemic PBMC RNA from 30 patients was extracted for target preparation and hybridization onto Affymetrix microarrays. We also isolated PBMCs and PBMCs subjected to enrichment for CD8+ cells from control patients. RNA from these cells (naÃ¯ve or activated with phytohemagglutinin) was extracted for target preparation and hybridization onto Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Norman Lee

PROVIDER: E-GEOD-10631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Molecular profiling of LGL leukemia reveals role of sphingolipid signaling of cytotoxic lymphocytes

Project description:We used microarrays to expression profile peripheral blood mononuclear cells (PBMCs) from LGL leukemia patients and control subjects to identify survival pathways that render leukemic LGL resistant to activation induced cell death. Keywords: granular lymphocyte leukemia, PBMC

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Involvement of hCMV in the ontogeny of CD4+ T-LGL

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miR181a is a novel key player in the STAT3 mediated survival network of TCRab CD8+ T-LGL leukemia [RNA-Seq]

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Transgenic NUP98-HOXA9 Zebrafish Embryos: Unactivated ('lGl') vs. Cre-activated ('NHA9')

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