DsbA plays a critical and multi-faceted role in the production of secreted virulence factors by Erwinia carotovora

ABSTRACT: Erwinia carotovora subsp. atroseptica (Eca) is an enterobacterial phytopathogen causing economically-significant soft rot disease. Pathogenesis is mediated by multiple secreted virulence factors, many of which are secreted by the Type II (Out) secretion system. DsbA catalyses the introduction of disulphide bonds into periplasmic and secreted proteins. In this study, the extracellular proteome (secretome) of wild type Eca SCRI1043 and dsbA and out mutants was analysed by spectral counting mass spectrometry. This revealed that dsbA inactivation had a huge impact on the secretome and identified diverse DsbA- and Out-dependent secreted proteins, representing known, predicted and novel candidate virulence factors. Further characterisation of the dsbA mutant showed that secreted enzyme activitie

ORGANISM(S): Pectobacterium carotovorum

SUBMITTER: Pete Hedley 

PROVIDER: E-GEOD-10647 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress