Transcription profiling of reduced folate carrier (RFC1-/-) mouse knockouts at E9.5 reveals altered gene expression of the cubulin-megalin complex

ABSTRACT: The reduced folate carrier (RFC1) is an integral membrane protein and facilitative anion exchanger that mediates delivery of 5-methyltetrahydrofolate into mammalian cells. Adequate maternal-fetal transport of folate is necessary for normal embryogenesis. Targeted inactivation of the murine RFC1 gene results in post-implantation embryo lethality, but daily folic acid supplementation of pregnant dams prolongs survival of homozygous embryos until mid-gestation. At E10.5 RFC1-/- embryos are developmentally delayed relative to wildtype littermates, have multiple malformations, including neural tube defects, and die due to failure of chorioallantoic fusion. The mesoderm is sparse and disorganized, and there is a marked absence of erythrocytes in yolk sac blood islands. Affymetrix microarray

ORGANISM(S): Mus musculus

SUBMITTER: Janee Gelineau-van Waes 

PROVIDER: E-GEOD-10659 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress