Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

23 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Methotrexate treatment-dynamics of Arabidopsis transcriptome during folate depletion

ABSTRACT: cea06-02_folate - methotrexate treatment - global analysis of the folate metabolism in Arabidopsis - Treatment of cells of Arabidopsis by methotrexate which is an inhibitor of the synthesis of the folate. Consequently, the pool of folates presents a strong reduction. The goal of this project is to look at the variations of expression in the Arabidopsis genome and specifically in some genes implied in the metabolism of the folate. Keywords: time course,treated vs untreated comparison 10 dye-swap - CATMA arrays

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Agnes Yu

PROVIDER: E-GEOD-10675 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

A genome-wide and metabolic analysis determined the adaptive response of Arabidopsis cells to folate depletion induced by methotrexate.

Loizeau Karen K De Brouwer Veerle V Gambonnet Bernadette B Yu Agnès A Renou Jean-Pierre JP Van Der Straeten Dominique D Lambert Willy E WE Rébeillé Fabrice F Ravanel Stéphane S

Plant physiology 20081017 4

Control of folate homeostasis is essential to sustain the demand for one-carbon (C1) units that are necessary for major biological functions, including nucleotide synthesis and methylation reactions. In this study, we analyzed the genome-wide and metabolic adaptive response of Arabidopsis (Arabidopsis thaliana) cells to folate depletion induced by the antifolate methotrexate. Drug treatment induced a response typical to xenobiotic stress and important changes in folate content and composition. T ...[more]

PMID: 18931140

Similar Datasets

rilkit-eqtl analysis (random pair design). Tools to optimize the use of RIL populations for natural diversity studies and QTL cloning

Project description:gnp07_rilkit - rilkit-eqtl analysis (random pair design). To provide quantitative geneticists studying natural variation in our RIL populations with information and resources necessary to speed up QTL cloning and candidate genes identification. This articulates around the creation of two major resources and their integration into databases to make the information accessible to users: A. Whole transcriptome survey, B. Production of complementary material. Comparison of 164 Cvi/Col(8RV) Recombinant Inbred Lines (RILs) and 164 Bur/Col (20RV) RILs. 162 dye-swaps. Genotype and ecotype comparison.

2011-06-30 | E-GEOD-28791 | biostudies-arrayexpress

eqtl analysis (random pair design)-Establishing the network of seed gene expression and analysis of its biodiversity

Project description:gnp3_tri33-arabidoseed - eqtl analysis (random pair design) - WP3 : Biodiversity of seed traits : state of the art - Comparison of the developping seed transcriptome in 160 Bay0/Sha Recombinant Inbred Lines (RILs) at 10 days after pollinisation. Keywords: genotype and ecotype comparison 80 dye-swap - CATMA arrays

2009-06-05 | E-GEOD-16393 | biostudies-arrayexpress

Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing

Project description:This SuperSeries is composed of the following subset Series: GSE24692: chip in silencing suppressor plants or in mirna plants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing GSE24693: gene profiling in silencing suppressor plants or in mirna mutants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing GSE26739: Expression profiling in silencing suppressor plants or in mirna mutants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing Refer to individual Series

2011-08-22 | E-GEOD-24837 | biostudies-arrayexpress

gene profiling in silencing suppressor plants or in mirna mutants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing

Project description:gen107_ptgs - gene profiling in silencing suppressor plants or in mirna mutants - What are the genes that are differentially regulated in various tissues derived from silencing suppressor plants or miRNA mutants? - HcPro, P15, P19, CHS-RNAi (control), were grown on MS solid medium, and tissues were harvested at different developmental stages. dcl1-9, hen1-1 and La-er (control) were grown on MS solid medium, and tissues harvested at different developmental stages. Keywords: gene knock in (transgenic),gene knock out 60 dye-swap - CATMA arrays

2011-08-22 | E-GEOD-24693 | biostudies-arrayexpress

Expression profiling in silencing suppressor plants or in mirna mutants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing

2011-08-22 | E-GEOD-26739 | biostudies-arrayexpress

Interactions between H2O2 and glutathione in gene expression

Project description:rs08-03_glutathion - glutathion - How does glutathione content or reduction state affect H2O2-induced changes in the transcriptome? - Three single Arabidopsis mutants were used: cat2, knockout for catalase2 and so enriched in H2O2; cad2, defective in glutathione content; cytGR, knockout for cytosolic glutathione reductase. Cat2 was crossed with cad2 and cyt GR, and col0, 3 single mutants, and 2 double mutants were sampled in controlled growth conditions either in 8h or 16h photoperiod. Keywords: gene knock out 20 dye-swap pairs - CATMA arrays: 40 arrays

2010-07-22 | E-GEOD-21424 | biostudies-arrayexpress

comparison of transcriptomes in mad mutants-Transcriptome analysis of miRNA action deficient mutants

Project description:rs10-04_mad - comparison of transcriptomes in mad mutants - What gene sets are differentially expressed in mad mutants? - Seeds of GFP171.1 (parental line), mad1, mad2, mad3, mad6 and dcl1-12 were sterilized and germinated on Murashige/Skoog medium with 0.9% agar. Plates were stratified at 4C in the dark for 4 days. The plates were then transferred to a growth cabinet at 21C under a 16h light/8h darkness light regime, and the seedlings were harvested 18 days after transfer to the growth cabinet. 10 dye-swap - genotype comparaison

2011-09-14 | E-GEOD-30477 | biostudies-arrayexpress

Differential expression in laccase mutants of Arabidopsis thaliana

Project description:ra10-01_laccases; laccase mutations. We demonstrated that laccases are involved in lignin polymerisation. Mutants have already been tested on microarrays and there is few differences compared to wild-type. The laccase mutation seems surgical. We possess a new double mutant, called snips, with a semi-dwarf phenotype, and we want to determine its profile. Each mutant was compared to wild type. All plants were harvested at the same developmental stage in the same growth chamber between 10h30 and 11h. 10 dye-swaps. CATMA arrays.

2011-07-02 | E-GEOD-22907 | biostudies-arrayexpress

compare haiku mutants with wildtype-Study of small seed mutants.

Project description:au07-11_haiku - compare haiku mutants with wildtype - What are other genes involved in haiku pathway? - IKU1, IKU2 belong to same pathway which involved in seed development. Using array analysis try to find other components of this pathway. Keywords: gene knock out 10 dye-swap - CATMA arrays

2009-07-08 | E-GEOD-16956 | biostudies-arrayexpress

kinetics of gene expression after light exposure in hub1-3 mutant compared to wild-type

Project description:au10-03_hub1 - kinetics of gene expression after light exposure in hub1-3 mutant compared to wild-type - Role of histone UbH2B in the regulation of gene expression in response to light - RNA from 5-day-old wild-type and hub1-3plants in 3 conditions were used for hybridization: 5 days of darkness, 5 days of darkness + 1 hour of light and 5 days of darkness + 6 hours of light. Keywords: genotype comparaison,time course 10 dye-swap - CATMA arrays

2011-05-20 | E-GEOD-21922 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data