Deep sequencing of small RNA libraries from chicken embryo

ABSTRACT: MicroRNA (miRNA) and other types of small regulatory RNAs play a crucial role in the regulation of gene expression in eukaryotes. Several distinct classes of small regulatory RNAs have been discovered in recent years. To extend the repertoire of small regulatory RNAs characterized in chickens we used a deep sequencing approach developed by Solexa (now Illumina Inc.). We sequenced three small RNA libraries prepared from different developmental stages of the chicken embryo (days 5, 7, and 9) to produce over 9.5 million short sequence reads. We developed a bioinformatics pipeline to distinguish authentic mature miRNA sequences from other classes of small RNAs and short RNA fragments represented in the sequencing data. Using this approach we detected almost all of the previously known chicken

ORGANISM(S): Gallus gallus

SUBMITTER: Evgeny Glazov 

PROVIDER: E-GEOD-10686 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress