Transcription profiling of human umbilical vein endothelial cells (HUVEC) cultured under retroviral transduction, on different culture substrates and in different geometries

ABSTRACT: Overexpression of a caspase-resistant form of Bcl-2 (D34A) in human umbilical vein endothelial cells (EC) implanted into immunodeficient mice promotes the maturation of human EC-lined microvessels invested by vascular smooth muscle cells (VSMC) of mouse origin. In contrast, EC implants not overexpressing Bcl-2 form only simple, uncoated EC tubes. Here we compare the phenotypes of vessels formed in vivo and the transcriptomes in vitro of EC expressing different forms of Bcl-2. Wild type Bcl-2, like the caspase resistant D34A Bcl-2 mutant, is anti-apoptotic in vitro and promotes VSMC recruitment in vivo, whereas a G145E mutant that has diminished anti-apoptotic activity in vitro does not promote vessel maturation in vivo. The D34A and wild type forms of Bcl-2, but not the G145E mutant form

ORGANISM(S): Homo sapiens

SUBMITTER: Cris Print 

PROVIDER: E-GEOD-10710 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress