Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

9 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from human colonic biopsy sample

ABSTRACT: Gene expression profile based classification of colonic diseases are suitable for identification of diagnostic mRNA expression patterns which can establish the basis of a new molecular biological diagnostic method Keywords: whole genomic expression Total RNA was extracted from colonic biosy samples CRC and hybridized on Affymetrix HGU133 Plus 2.0 microarrays

ORGANISM(S): Homo sapiens

SUBMITTER: Norbert Solymosi

PROVIDER: E-GEOD-10714 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Reversal of gene expression changes in the colorectal normal-adenoma pathway by NS398 selective COX2 inhibitor.

Galamb O O Spisák S S Sipos F F Tóth K K Solymosi N N Wichmann B B Krenács T T Valcz G G Tulassay Z Z Molnár B B

British journal of cancer 20100119 4

<h4>Background and aims</h4>Treatment of colorectal adenomas with selective cyclooxygenase-2 inhibitors can contribute to the chemoprevention of colorectal cancer (CRC), but the molecular background of their effect is not fully understood. We analysed the gene expression modulatory effect of N-(2-cyclohexyloxy-4-nitrophenyl)-methanesulfonamide (NS398) on HT29 cells to be correlated with expression data gained from biopsy samples.<h4>Methods</h4>HT29 colon adenocarcinoma cells were treated with N ...[more]

PMID: 20087348

Publication: 1/2

Similar Datasets

Expression data from human colonic biopsy samples (adenoma-carcinoma)

Project description:Whole genomic microarray analysis was performed in order to identify gene expression profile alterations focusing on the dysplastic adenoma-carcinoma transition. Our aims were to determinate characteristic transcript sets for developing diagnostic mRNA expression patterns for objective classification of benign and malignant colorectal diseases and to test the classificatory power of these markers on an independent sample set. Total RNA was extracted from colonic biopsy samples of histologically negative patients and of patients with adenoma or colorectal cancer and were hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2013-01-01 | E-GEOD-37364 | biostudies-arrayexpress

Expression data from human colonic biopsy samples

Project description:Age-related transcriptome changes can contribute to better understanding of gastrointestinal disorders such as colorectal cancer in older population Total RNA was extracted from colonic biopsy samples from histologically normal children and the samples were hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2013-01-01 | E-GEOD-37267 | biostudies-arrayexpress

Expression data from human peripheral blood samples

Project description:Gene expression profile based classification of colonic diseases are suitable for identification of diagnostic mRNA expression patterns which can establish the basis of a new molecular biological diagnostic method. Keywords: whole genomic expression Total RNA was extracted from peripheral blood samples Diverticulitis and Infective colitis were hybridized on Affymetrix HGU133 Plus 2.0 microarrays.

2011-03-01 | E-GEOD-21826 | biostudies-arrayexpress

Expression data from human colonic epithelial cells normal (N), adenoma (AD) or colorectal cancer (CRC) tissues

Project description:The whole-genome oligonucleotide microarray analysis of laser microdissected human colonic epithelial cells can contribute to determination of disease-specific expression alterations in colonic epithelial cells and to localize the origin the expression changes measured in whole biopsy samples. Keywords: whole genomic expression Total RNA was extracted from laser microdissected human colon epithelial cells and hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2010-03-15 | E-GEOD-15960 | biostudies-arrayexpress

Expression data from human HT-29 immortalized colorectal adenocarcinoma cell line

Project description:Gene expression profiling for identification of genes regulated by DNA methylation Total RNA was extracted from HT-29 cell line was hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2012-01-01 | E-GEOD-29060 | biostudies-arrayexpress

Expression data from human HT29, HDFa cell lines after normal and tumor tissue derived DNA treatment

Project description:Gene expression profile based on human HT29, HDFa cell lines after normal and tumor tissue derived DNA treatment Total RNA was extracted from human HT29, HDFa cell lines after normal and tumor tissue derived DNA treatment were hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2015-06-11 | E-GEOD-67557 | biostudies-arrayexpress

Transcription profiling of peripheral blood samples from patients with Crohn's disease (CD) and Ulcerative colitis (UC) .

Project description:The whole-genome oligonucleotide microarray analysis of peripheral blood samples can contribute to the determination of distant blood markers of local pathophysiological alterations in colorectal diseases. These markers can lead to alternative screening procedures. Total RNA was extracted from peripheral blood samples of patients with Ulcerative colitis (UC) and Crohn's disease (CD) and hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2009-05-19 | E-GEOD-11499 | biostudies-arrayexpress

Expression data from normal and CRC human peripheral blood samples

Project description:The whole-genome oligonucleotide microarray analysis of peripheral blood samples can contribute to the determination of distant blood markers of local pathophysiological alterations in colorectal diseases. These markers can lead to alternative screening procedures. Keywords: whole genomic expression Total RNA was extracted from peripheral blood samples of healthy patients and patients with CRC and hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2009-01-29 | E-GEOD-10715 | biostudies-arrayexpress

Expression data from NS398-treated and control HT29 colon adenocarcinoma cell line samples

Project description:The whole-genome oligonucleotide microarray analysis of NS398-treated HT29 colon adenocarcinoma cells samples can give an insight into global molecular background of selective COX2 inhibitor administration in order to find other target molecules and pathways influenced by NS398 selective COX2 inhibitor treatment in the epithelial cells. Total RNA was extracted from NS398-treated and control HT29 colon adenocarcinoma cells and hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2010-03-16 | E-GEOD-15799 | biostudies-arrayexpress

Expression data from human liver cell line induced by PCB153

Project description:Exposure to Polychlorobiphenyls (PCBs) is known to cause serious health effects in human but the gene expression profiles leading to development of differnet diseases is not fully understood. The knowledge of global gene expression will help us to devlop early diagnostic biomarkers for PCB induced health effects. We used microarrays to detail the global gene expression profile underlying the effects of PCB153 on human liver cells leading to identification of distinct classes of up-regulated and down-regulated genes and cellular processes. Keywords: time course The HepG2 cells were grown for 48 hours in DMEM supplemented with 10% FBS and 1X Pennicillin-Streptromycin and treated with PCB153 for different times. Trizol extraction of total RNA was performed according to the manufacturer's instructions. Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microgram of total RNA (Expression Analysis Technical Manual, 2001, Affymetrix). Following fragmentation, 10 microgram of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HGU133 plus 2.0). GeneChips were washed and stained in the Affymetrix Fluidics Station 450 and scanned using the Affymetrix GeneArray Scanner 3000. The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.

2010-07-21 | E-GEOD-6494 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data