Transcription profiling of Drosophila cell lines: RasV12, four samples of CL8 and one sample of L2 reveal expression of oncogenic ras allows efficient generation of continuous cell lines

ABSTRACT: Analysis of cells in culture has made substantial contributions to biological research. The versatility and scale of in vitro manipulation and new applications such as high throughput gene silencing screens ensure the continued importance of cell-culture studies. In comparison to mammalian systems, Drosophila cell culture is underdeveloped, primarily because there is no general genetic method for deriving new cell lines. Here we found expression of the conserved oncogene, RasV12 (a constitutively activated form of Ras), profoundly influences the development of primary cultures derived from embryos. The cultures become confluent in about three weeks and can be passaged with great success. The cells have undergone more than 90 population doublings and therefore constitute continuous cell lin

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Amanda Simcox 

PROVIDER: E-GEOD-10781 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress