Transcription profiling of endothelial cells (EC) isolated from the unique vasculature of the corpus cavernosum to provide a human cavernosal endothelial cell phenotype

ABSTRACT: Purpose: To identify the molecular phenotype of endothelial cells (EC) isolated from the unique vasculature of the corpus cavernosum. Methods: Human EC derived from corpus cavernosum (HCCEC, n=5), coronary artery (HCAEC, n=4) and umbilical vein (HUVEC, n=3) were grown in culture and mRNA transcripts quantified by Affymetrix GeneChip microarrays. Genes differentially expressed across samples were partitioned around medoids to identify genes with highest expression in HCCEC. Several genes were verified by real-time PCR. The role of claudin 11 (CLDN11) in endothelial cell barrier function was examined by in vitro transendothelial electrical resistance assay. Results: Unsupervised hierarchical clustering of samples using all expressed genes yielded discrete groupings of EC lines according to t

ORGANISM(S): Homo sapiens

SUBMITTER: Hunter Wessells 

PROVIDER: E-GEOD-10804 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress