Microarray analysis to identify novel genes regulated by Cholera toxin in bone marrow-derived dendritic cells (BMDCs)

ABSTRACT: The mechanisms by which dendritic cells (DCs) induce differentiation of naïve CD4+ T cells along the Th2 lineage is not well understood given that DCs themselves do not produce IL-4. In the present study, we undertook a microarray approach to identify genes involved in the induction of Th2 differentiation by DCs treated with a Th2-skewing adjuvant cholera toxin (CT). In the microarray analysis, murine bone marrow derived immature DCs were treated with CT. Of particular interest was tthe significant upregulation of the expression of c-kit. The upregulation of c-kit on DCs is critical for the induction of a Th2 response. Keywords: cholera toxin, bone marrow derived dendritic cells, gene expression array-based (RNA / spotted DNA/cDNA) Murine bone marrow cells were cultured in the presence o

ORGANISM(S): Mus musculus

SUBMITTER: Anuradha Ray 

PROVIDER: E-GEOD-10815 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress