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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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High-throughput sequencing of small RNAs from HeLa cells

ABSTRACT: High-throughput sequencing of small RNAs from HeLa cells Keywords: high-throughput Solexa sequencing Small RNAs were sequenced from HeLa cells

ORGANISM(S): Homo sapiens

SUBMITTER: wei chen

PROVIDER: E-GEOD-10829 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Discovering microRNAs from deep sequencing data using miRDeep.

Friedländer Marc R MR Chen Wei W Adamidi Catherine C Maaskola Jonas J Einspanier Ralf R Knespel Signe S Rajewsky Nikolaus N

Nature biotechnology 20080401 4

The capacity of highly parallel sequencing technologies to detect small RNAs at unprecedented depth suggests their value in systematically identifying microRNAs (miRNAs). However, the identification of miRNAs from the large pool of sequenced transcripts from a single deep sequencing run remains a major challenge. Here, we present an algorithm, miRDeep, which uses a probabilistic model of miRNA biogenesis to score compatibility of the position and frequency of sequenced RNA with the secondary str ...[more]

PMID: 18392026

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