Tumor Liver vs Normal Liver: ChIP-chip and RNA expression

ABSTRACT: There is widespread interest in efficient characterization of differences between tumor and normal samples. Here, we demonstrate an effective methodology for genome-scale characterization of tumors. Using matched normal and tumor samples from liver cancer patients, as well as non-cancer-related normal liver tissue, we first determined changes in gene expression as monitored on RNA expression arrays. We identified several hundred mRNAs that were consistently changed in the tumor samples. To characterize the mechanisms responsible for creation of the tumor-specific transcriptome, we performed ChIP-chip experiments to assay binding of RNA polymerase II, H3me3K27, and H3me3K9 in 25,000 promoter regions. These experiments identified changes in active and silenced regions of the genome in the

ORGANISM(S): Homo sapiens

SUBMITTER: Peggy Farnham 

PROVIDER: E-GEOD-10842 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress