Transcription profiling of rice root tips before, at and buckled by a hard layer in two rice varieties

ABSTRACT: The aim of this study was to determine the changes in gene expression of rice root tips when they came in to contact with a hard layer (60% wax layer). Three categories of root tips were sampled; tips before the hard layer, tips that had come into contact with the hard layer and root tips which had buckled after coming into contact with the hard layer. Two genotypes (Azucena and Bala) that vary in there ability to penetrate a hard layer were selected for a genotype comparison of gene expression at the hard layer. Experiment Overall Design: A chamber with the internal dimensions of 10 x 5 x 25cm consisting of a glass back, Perspex front and aluminium spacers with an open bottom and top was set at an angle of 15o in a large sand filled tray and filled up to 19.5 cm with dry sand. The sand wa

ORGANISM(S): Oryza sativa

SUBMITTER: Gareth Norton 

PROVIDER: E-GEOD-10857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress