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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse uterus from OVX/oil/6h/UTX 129 WT vs. Hoxa-10-/- animals

ABSTRACT: Female mice used were SvJ/129, 10-16 weeks of age; Hoxa-10+/+ and Hoxa-10-/- littermates were used in parallel experiments. 14 days after ovariectomy, mice were injected with sesame oil; (Sigma; 0.1mL/mouse), followed by sacrifice and removal of uterine; horns at 6 hours after injection. 8 uterine horns from 4 mice were; pooled to form one sample. Total RNA preparation, cRNA labeling and; hybridization were performed according to recommendations by; Affymetrix. All 6 samples were collected and processed in parallel.

ORGANISM(S): Mus musculus

SUBMITTER: Richard Maas

PROVIDER: E-GEOD-109 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiling reveals progesterone-mediated cell cycle and immunoregulatory roles of Hoxa-10 in the preimplantation uterus.

Yao Mylene W M MW Lim Hyunjung H Schust Daniel J DJ Choe Sung E SE Farago Anna A Ding Yueyun Y Michaud Sebastien S Church George M GM Maas Richard L RL

Molecular endocrinology (Baltimore, Md.) 20030109 4

Human infertility and recurrent pregnancy loss caused by implantation defects are poorly understood. Hoxa-10-deficient female mice have severe infertility and recurrent pregnancy loss due to defective uterine implantation. Gene expression profiling experiments reveal that Hoxa-10 is an important regulator of two critical events in implantation: stromal cell proliferation and local immunosuppression. At the time of implantation, Hoxa-10 mediates the progesterone-stimulated proliferation of uterin ...[more]

PMID: 12554760

Similar Datasets

Transcription profiling of uterine horns from Hoxa-10+/+ and Hoxa-10-/- SvJ/129 mice treated with or without progesterone to study the progesterone-mediated roles of Hoxa-10 in preimplantation uterus.

Project description:This SuperSeries is composed of the following subset Series:; GSE108: OVX/P4/6h/UTX 129 WT vs. Hoxa-10-/-; GSE109: OVX/oil/6h/UTX 129 WT vs. Hoxa-10-/- Experiment Overall Design: Refer to individual Series. Female mice used were SvJ/129, 10-16 weeks of age; Hoxa-10+/+ and Hoxa-10-/- littermates were used in parallel experiments. 14 days after ovariectomy, mice were injected subcutaneously with progesterone dissolved in sesame oil (Sigma; 0.2mg/0.1mL/mouse) or sesame oil (Sigma; 0.1mL/mouse), followed by sacrifice and removal of uterine horns at 6 hours after injection. 8 uterine horns from 4 mice were pooled to form one sample. Total RNA preparation, cRNA labeling and hybridization were performed according to recommendations by Affymetrix.

2007-11-14 | E-GEOD-6439 | biostudies-arrayexpress

Transcription profiling of mouse uterine horns from Hoxa-10+/+ and Hoxa-10-/- littermates

Project description:Female mice used were SvJ/129, 10-16 weeks of age; Hoxa-10+/+ and Hoxa-10-/- littermates were used in parallel experiments. 14 days after ovariectomy, mice were injected subcutaneously with progesterone dissolved in sesame oil (Sigma; 0.2mg/0.1mL/mouse), followed by sacrifice and removal of uterine horns at 6 hours after injection. 8 uterine horns from 4 mice were pooled to form one sample. Total RNA preparation, cRNA labeling and hybridization were performed according to recommendations by Affymetrix. The 6 samples were obtained and processed in two batches: (1: GSM3047 and GSM3041), and (2: GSM3048, GSM3049, GSM3042, and GSM3043).

2007-10-06 | E-GEOD-108 | biostudies-arrayexpress

Transcription profiling of uterine horns from SvJ/129 mice treated with progesterone to study the progesterone-mediated roles of Hoxa-10 in preimplantation uterus.

Project description:This SuperSeries is composed of the following subset Series: GSE106: OVX/P4/UTX 129/SvImJ WT A; GSE107: OVX/P4/UTX 129/SvImJ WT B Experiment Overall Design: Refer to individual Series. Female mice used were 129/SvImJ wildtype (Jackson Lab), 10-16 weeks of age. 14 days after ovariectomy, mice were injected subcutaneously with progesterone dissolved in sesame oil (Sigma; 0.2mg/0.1mL/mouse), followed by sacrifice and removal of uterine horns at various times after injection. 8 uterine horns from 4 mice were pooled to form one sample. Total RNA preparation, cRNA labeling and hybridization were performed according to recommendations by Affymetrix.

2007-11-15 | E-GEOD-6440 | biostudies-arrayexpress

Transcription profiling of uterus from 129/SvImJ wildtype mice treated with progesterone

Project description:Female mice used were 129/SvImJ wildtype (Jackson Lab), 10-16 weeks; of age. 14 days after ovariectomy, mice were injected; subcutaneously with progesterone dissolved in sesame oil (Sigma;; 0.2mg/0.1mL/mouse), followed by sacrifice and removal of uterine; horns at various times after injection. 8 uterine horns from 4 mice were; pooled to form one sample. Total RNA preparation, cRNA labeling and; hybridization were performed according to recommendations by; Affymetrix.

2008-06-11 | E-GEOD-106 | biostudies-arrayexpress

Transcription profiling of mouse uterine horns from OVX/P4/UTX 129/SvImJ WT B

2008-06-11 | E-GEOD-107 | biostudies-arrayexpress

Effects of estrogen and progesterone on glycosyltransferase expression in mice

Project description:This laboratory studies the structural basis of carbohydrate function in terms of adhesion and other biological processes. The Fisher lab is testing the hypothesis that the mucin-coated oral and uterine cavities present similar carbohydrate receptors that specify the bacterial ecology of both regions and the repertoire of these oligosaccharide species is hormonally regulated. This theory also suggests that certain individuals express carbohydrate receptors that make them susceptible to both periodontal disease and preterm labor. Experimental procedure: 40 mature female mice were ovariectomize . The mice were allowed to rest for two weeks to eliminate any remaining endogenous estrogen (E2) and progesterone (P4). Then the mice were separated into four groups (10 each) that received the treatment indicated. Group 1: ovariectomized mice with no supplement, only sesame oil vehicle. Group 2: ovariectomized mice with only P4 supplement (2 mg/day/mouse). Group 3: ovariectomized mice with E2 supplement (100 ng/day/mouse). Group 4: ovariectomized mice with both P4 (2 mg/day/mouse) and E2 (100 ng/day/mouse) supplement. The treatment lasted for 4 days; the steroids were dissolved in sesame oil and injected subcutaneously. After treatment, salivary glands (parotid, submandibular, and sublingual gland), submandibular lymph nodes and uterine horns with cervix were collected. Total RNA was extracted from these samples using Trizol.

2011-03-02 | E-GEOD-27598 | biostudies-arrayexpress

Potential mechanisms of endocrine disruptors as revealed by uterine gene profile

Project description:Females were ovariectomized and injected with sesame oil, estradiol in sesame oil, BPA in sesame oil or HPTE in sesame oil. Uterine tissue was collected after 2 or 24 hours. RNA was analyzed by microarray compare ealry and late responses to a potent and a weak estrogen agaonist.

2010-10-07 | GSE24525 | GEO

Changes in Mouse Uterine Lumenal Epithelial Cell Transcriptome in Estrus Compared to Proestrus

Project description:The potential for xenoestrogens and other environmental chemicals to alter female reproductive function calls for the study of their affects on endogenous hormone-regulated gene expression pathways during uterine tissue remodeling which occurs as part of the menstrual/estrus cycle. However, our knowledge of these pathways is limited. Here, we characterize changes in the CD-1 mouse uterine luminal epithelial cell transcriptome during proestrus and estrus, which are regulated by estrogen and progesterone in preparation of the uterus for pregnancy. Mice were staged beginning at 6 wk of age and uterine horns were harvested at estrus and proestrus. RNA was extracted from lumenal epithelium of uterine horns. Microarray analysis identified 2,251 genes differentially expressed in estrus compared to proestrus in lumenal epithelial cells. These complement other studies where RNA was extracted from whole uterine horns of mice in estrus and proestrus (GSE43064).

2013-06-07 | GSE46813 | GEO

Do differences in the endometrial transcriptome between uterine horns ipsilateral and contralateral to the corpus luteum influence conceptus growth to Day 14 in cattle?

Project description:This study relates to embryo-maternal interaction. The aim was to compare the transcriptome and ability of the ipsilateral and contralateral uterine horns to support preimplantation conceptus survival and growth to Day 14. Although differences in gene expression exist between the endometrium of uterine horns ipsilateral and contralateral to the CL in cattle, they do not impact conceptus survival or length between Days 7 and 14.

2018-08-15 | GSE118507 | GEO

Deletion of the Hoxa Cluster in MLL-AF9 Is Incompatible with Leukemia Maintenance: RNAseq-based Quantitative Analysis of Wild Type and Hoxa-del Transcriptomes

Project description:High HOXA expression correlates with poor clinical outcome in AML, particularly those harboring MLL rearrangements (MLLr). The necessity of the HOXA cluster for the maintenance of MLLr-leukemia has not been elucidated. Primary leukemias were generated by transduction of MLL-AF9 (MA9) into hematopoietic stem and progenitor cells from compound Cre responsive transgenic mice for conditional deletion of the Hoxa locus. Hoxa deletion resulted in reduced proliferation, colony formation and repopulating ability in transplanted mice in which surviving leukemic cells retained at least one copy of the Hoxa cluster (Hoxa-del). Comparative RNA-seq analysis of leukemic MA9-Hoxa-wild type (WT) and MA9-Hoxa-del cells identified a unique gene signature.

2021-04-16 | GSE113200 | GEO

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