Transcription profiling of human formalin-fixed paraffin-embedded breast cancer core biopsies

ABSTRACT: The routine workflow for invasive cancer diagnostics is based on biopsy processing by formalin fixation and subsequent paraffin embedding. Formalin-fixed paraffin-embedded (FFPE) tissue samples are easy to handle, stable and particularly suitable for morphologic evaluation, immunohistochemistry and in situ hybridization. However, it has become a paradigm that these samples cannot be used for genome-wide expression analysis with microarrays. To oppose this view, we present a pilot microarray study using FFPE core needle biopsies from breast cancers as RNA source. We found that microarray probes interrogating sequences near the poly-A-tail of the transcribed genes were well suitable to measure RNA levels in FFPE core needle biopsies. For the ER and the HER2 gene, we observed strong correlat

ORGANISM(S): Homo sapiens

SUBMITTER: Jan Budczies 

PROVIDER: E-GEOD-11001 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress