Genome-wide transcriptional profiling of Lactobacillus acidophilus' response to bile

ABSTRACT: Lactobacillus acidophilus NCFM is an industrially important strain used extensively as a probiotic culture. Tolerance of the presence of bile is an attribute important to microbial survival in the intestinal tract. A whole-genome microarray was employed to examine the effects of bile on the global transcriptional profile of this strain, with the intention of elucidating genes contributing to bile tolerance. Genes involved in carbohydrate metabolism were generally induced, while genes involved in other aspects of cellular growth were mostly repressed. A 7-kb eight-gene operon encoding a two-component regulatory system (2CRS), a transporter, an oxidoreductase, and four hypothetical proteins was significantly upregulated in the presence of bile. Deletion mutations were constructed in six gene

ORGANISM(S): Lactobacillus acidophilus NCFM

SUBMITTER: Todd Klaenhammer 

PROVIDER: E-GEOD-11048 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress