Sub1 occupancy in yeast cells in exponential growth condition

ABSTRACT: A genome-wide location analysis by ChIP on chip of the gene occupancy by Sub1 was undertaken to define the gene targets of Sub1 in vivo. Chromatin immunoprecipitation (ChIP) assays were performed on epitope-tagged Sub1-3HA cross-linked chromatin from exponentially growing cells. Immunopurified DNA and DNA from whole-cell extracts were fluorescently labelled and competitively hybridized to DNA microarrays harbouring ORFs and intergenic regions. The ratio of fluorescence intensities at each site in the microarray provided a measure of the extent of Sub1 binding to a specific genomic locus. Data from three independent experiments were compiled. Many loci were found to be significantly enriched in active growth conditions where Sub1 is not essential for cell viability. Approximately one-fourth

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Christine Conesa 

PROVIDER: E-GEOD-11054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress