Experiment to determine DNA copy number of the HCE-T cell line relative to normal duploid cell

ABSTRACT: Purpose. Simian virus 40 (SV40)-immortalized human corneal epithelial (HCE-T) cells have been widely used as an in vitro model of human corneal epithelial cells. To better understand the nature of this cell line, we assessed it for genomic aberrations and cellular heterogeneity. Methods. For the quantitative measurement of genomic aberrations, array based comparative genomic hybridization (CGH) analysis was performed. For identification of cellular heterogeneity, cell morphology, growth kinetics, trans-epithelial electrical resistance and transcriptional efficiency were analyzed. We also carried out real-time PCR and chromosomal fluorescent in situ hybridization (cFISH) against some gained or lost loci in order to assess genomic heterogeneity. To assess differences in the gene expression p

ORGANISM(S): Homo sapiens

SUBMITTER: Satoshi Kawasaki 

PROVIDER: E-GEOD-11101 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress